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Product Details
Synonyms
FAP, FAPalpha, SIMP, Seprase, APCE
Source
Human FAP, His Tag (FAP-H5244) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 86.9 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human FAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FAP, His Tag (Cat. No. FAP-H5244) is more than 95% and the molecular weight of this protein is around 180-220 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human FAP, His Tag (Cat. No. FAP-H5244) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-FAP (Sibrotuzumab) Antibody, Human IgG1 (AM111) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human FAP, His Tag (Cat. No. FAP-H5244) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a (AM108) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-FAP Antibody, Mouse IgG2a (FAP5) captured on CM5 chip via anti-mouse antibodies surface can bind Human FAP, His Tag (Cat. No. FAP-H5244) withan affinity constant of 9.47 nM asdetermined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >8000 pmol/min/µg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Prolyl endopeptidase FAP
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
88 Details
- Highest Development Stage：
Phase 3 Clinical
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