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Product Details
Synonyms
FCER1A, FCE1A, FcERI
Source
Human Fc epsilon RI alpha, His Tag (FCA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Gln 205 (Accession # P12319-1).
Predicted N-terminus: Val 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.9 kDa. The protein migrates as 37-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Fc epsilon RI alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) is more than 95% and the molecular weight of this protein is around 37-58 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Immunoglobulin E, Human Plasma at 5 μg/mL (100 μL/well) can bind Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) immobilized on CM5 Chip can bind Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3) with an affinity constant of 0.391 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) on AR2G Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 2.71 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
IgE Fc receptor, alpha-subunit
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Approved
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