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Product Details
Synonyms
FcRn, FCGRT & B2M
Source
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (FCM-C52W8) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Pro 298 (FCGRT) & Val 28 - Asn 125 (B2M) (Accession # XP_038512242.1 (FCGRT) & NP_001271408.1 (B2M)).
Predicted N-terminus: Ala 23 (FCGRT) & Val 28 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-37 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 0.171 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.132 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 1.11 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
IgG receptor FcRn large subunit p51
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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