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Product Details
Synonyms
FcRn, FCGRT & B2M
Source
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (FCM-M82W5) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-55 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 3.42 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 2.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
C***n2023-05-03
LikesI used Acro's FcRn for development of novel assays. The proteins within just a few days of placing the order. The proteins were also high quality, stable, and did not result in any background in any of the assays that were developed, even after using them for months. These produced great data and we have continued to repurchase them since. I would highly recommend this product.
Background
Recent Advances
Drug Development Progress- English Name：
IgG receptor FcRn large subunit p51
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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