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Product Details
Synonyms
LGALS1, GAL1, GBP, Galectin-1, DKFZp686E23103
Source
Human Galectin-1, Tag Free (GA1-H4113) is expressed from E. coli cells. It contains AA Met 1 - Asp 135 (Accession # P09382-1).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 14.7 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Galectin-1, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Galectin-1, Tag Free (Cat. No. GA1-H4113) is more than 95% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Mouse CD45 protein, His Tag (Cat. No. CD5-M52H3) captured on CM5 chip via anti-His antibody can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 7.19 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD45, His Tag (Cat. No. CD5-H52H8) on HIS1K Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 3.0 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human CD45, His,Avitag (Cat. No. CD5-H82E7) on SA Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 2.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
L***h2021-12-13
LikesDear Sir or Madam, I had an overall good service from Acro Biosystems. Shipment was fast and product quality was good. Got the protocol for QC after asking the customer service. Protocol for QC was easy to understand and repeat. Easy way to download the Certificate of Analysis via the printed QR code on product vials. Customer service answered very fast and was very friendly. QC of Human Galectin-1 protein vs. biotinylated human CD45 protein (CD5-H82E7) was fine.
Background
Recent Advances
Drug Development Progress- English Name：
Galectin-1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Approved
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