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Human Galectin-9 / LGALS9 Protein, His Tag, low endotoxin

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Cat. No. / Size
Price
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LG9-H5244-50ug
$315.00
LG9-H5244-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    Galectin-9, LGALS9, Ecalectin

  • Source

    Human Galectin-9, His Tag (LG9-H5244) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Thr 323 (Accession # O00182-2).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    Galectin-9 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 36.6 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Galectin-9 SDS-PAGE

    Human Galectin-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Galectin-9 ELISA

    Immobilized Human Galectin-9, His Tag (Cat. No. LG9-H5244) at 1 μg/mL (100 μL/well) can bind Recombinant Antibody Service-Anti-Gal9 Antibody,Human IgG4 L445P-Kappa with a linear range of 0.06-4 ng/mL (Routinely tested).

    Protocol

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Background

Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

Recent Advances

 
Drug Development Progress
  • English Name:

    Galactoside-binding lectin Galectin 9

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Phase 2 Clinical

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