Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
GLB-V82E3-100ug
$399.00
GLB-V82E3-1mg
$2667.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glycoprotein B/gB (HSV-2)

  • Source

    Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (GLB-V82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Ala 730 (Accession # P06763).

    Predicted N-terminus: Glu 98

    Request for sequence
  • Molecular Characterization

    Glycoprotein B/gB (HSV-2) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 76.0 kDa. The protein migrates as 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a trimer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glycoprotein B/gB (HSV-2) SDS-PAGE

    Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glycoprotein B/gB (HSV-2) SEC-MALS

    The purity of Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) is more than 90% and the molecular weight of this protein is around 235-270 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Glycoprotein B/gB (HSV-2) ELISA

    Immobilized Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse anti-Herpes virus 1 and 2, clone T111, Mouse IgG1 with a linear range of 0.02-1 ng/mL (QC tested).

    Protocol

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Background

Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress. Also plays a role, together with gK, in virus-induced cell-to-cell fusion (syncytia formation).

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