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Product Details
Synonyms
Glycoprotein C/Gc protein (HTNV)
Source
Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag (PRN-V52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 649 - Asn 1105 (Accession # P08668).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 52.5 kDa. The protein migrates as 54-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag (Cat. No. PRN-V52H4) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag (Cat. No. PRN-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (6D5) (Cat. No. PRN-MY322) with a linear range of 0.05-2 ng/mL (Routinely tested).
Protocol
Immobilized Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag (Cat. No. PRN-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (3G10) (Cat. No. PRN-MY323) with a linear range of 0.05-0.8 ng/mL (Routinely tested).
Protocol
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FAQ
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What do “pre” and “post” refer to in the names of viral proteins?
In virology, “pre” refers to the conformation of a viral surface glycoprotein before fusion with the host cell membrane, while “post” refers to the conformation after membrane fusion and entry into the host cell. Both are naturally occurring states in the viral life cycle. Because the “pre” conformation represents a critical stage before viral entry, antibodies designed against this state often exhibit stronger neutralizing activity and are therefore more effective in preventing infection.
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