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Product Details
Synonyms
Glycoprotein G (HeV)
Source
Hendra virus Glycoprotein, His Tag (GLN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 71 - Ser 604 (Accession # O89343-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 61.5 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.3 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Hendra virus Glycoprotein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Hendra virus Glycoprotein, His Tag (Cat. No. GLN-H52H3) is more than 85% and the molecular weight of this protein is around 70-100 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Hendra virus Glycoprotein, His Tag (Cat. No. GLN-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag (Cat. No. EPN-H5259) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Hendra virus Glycoprotein, His Tag (Cat. No. GLN-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-Nipah/Hendra Glycoprotein G Antibody, Human IgG1 with a linear range of 0.03-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Nipah/Hendra Glycoprotein G Antibody, Human IgG1 captured on Protein A Chip can bind Hendra virus Glycoprotein, His Tag (Cat. No. GLN-H52H3) with an affinity constant of 12.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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