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Product Details
Synonyms
Glycoprotein N/Gn protein (SFTSV)
Source
SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (GNN-S52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Lys 452 (Accession # F1BA47-1).
Predicted N-terminus: Asp 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.1 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
SFTS virus Gn Protein (Human/China/HB29/2010), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (Cat. No. GNN-S52H3) is more than 85% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (Cat. No. GNN-S52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SFTS-Gn Antibody, Human IgG1 (1G12) (Cat. No. SFN-M763) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (Cat. No. GNN-S52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SFTS-Gn Antibody, Human IgG1 (8F9) (Cat. No. SFN-M764) with a linear range of 0.03-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-SFTS-Gn Antibody, Human IgG1 (1G12) (Cat. No. SFN-M763) captured on Protein A Chip can bind SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (Cat. No. GNN-S52H3) with an affinity constant of 3.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-SFTS-Gn Antibody, Human IgG1 (8F9) (Cat. No. SFN-M764) captured on Protein A Chip can bind SFTS virus Gn Protein (Human/China/HB29/2010), His Tag (Cat. No. GNN-S52H3) with an affinity constant of 0.482 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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