Hantaan virus (Orthohantavirus andesense) Gn Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
GNN-V52H5-50ug
$470.00
GNN-V52H5-100ug
$798.00
GNN-V52H5-1mg
$2940.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Gn protein, glycoprotein polyprotein, Phlebovirus_G1, Phlebovirus_G2

  • Source

    Hantaan virus (Orthohantavirus andesense) Gn Protein, His Tag (GNN-V52H5) is expressed from human 293 cells (HEK293). It contains AA Met 19 - Glu 374 (Accession # Q9E006).

    Predicted N-terminus: Met 19

    Request for sequence
  • Molecular Characterization

    Gn protein Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.0 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >0.95 as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Gn protein SDS-PAGE

    Hantaan virus (Orthohantavirus andesense) Gn Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 0.95 (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Gn protein SEC-MALS

    The purity of Hantaan virus (Orthohantavirus andesense) Gn Protein, His Tag (Cat. No. GNN-V52H5) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

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Background

Hantaviruses (genus Orthohantavirus) are enveloped, negative-sense, single-stranded ribonucleic acid (RNA) viruses. The genomes of hantaviruses are composed of tri-segmented single-stranded, negative sense RNA, designated as small (S), medium (M) and large (L) segments. Each segment encodes nucleocapsid protein (NP), glycoprotein precursor (GPC) and RNA-dependent RNA polymerase (RdRp), respectively. GPC is posttranslationally cleaved into Gn and Gc. Glycoproteins Gn and Gc are transmembrane proteins and constitute an envelope with a lipid membrane derived from host cells. Gn and Gc are involved in receptor binding, membrane fusion and induction of protective immunity. The two glycoproteins are presumed to be the major elements involved in the induction of neutralizing antibodies during hantavirus infection.

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