PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

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Cat. No. / Size
Price
Qty
HLA-HP2H5-25ug
$410.00
HLA-HP2H5-200ug
$2350.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    HLA-A*0201 & B2M & AFP (FMNKFIYEI)

  • Source

    PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein (HLA-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FMNKFIYEI peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & FMNKFIYEI).

    Predicted N-terminus: Gly 25 & Ile 21

    Request for sequence
  • Molecular Characterization

    PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is assembled by biotinylated monomer (HLA-H82E3) and PE-labeled streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein is produced by co-expression of HLA and B2M loaded with AFP peptide. Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     HLA-A*0201 & B2M & AFP (FMNKFIYEI) ELISA

    Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein (Cat. No. HLA-HP2H5) at 1 μg/mL (100 μL/well) can bind Anti-HLA-A2&AFP Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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Background

Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms and binds copper, nickel, fatty acids, and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. The Human HLA-A*0201 AFP (FMNKFIYEI) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and FMNKFIYEI peptide of the AFP.

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