PE-Labeled Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein

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Cat. No. / Size
Price
Qty
HLV-HP2E4-25ug
$410.00
HLV-HP2E4-200ug (100ug X 2)
$2350.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    HLA-A*0201 & B2M & HBV (FLLTRILTI)

  • Source

    PE-Labeled Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein (HLV-HP2E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FLLTKILTI peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & FLLTKILTI).

    Predicted N-terminus: Gly 25 & Ile 21

    Request for sequence
  • Molecular Characterization

    PE-Labeled Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein is assembled by biotinylated monomer and PE-labeled streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Complex Protein is produced by co-expression of HLA-A*02:01 and B2M loaded with HBV peptide. Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, 50-150 mM NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     HLA-A*0201 & B2M & HBV (FLLTRILTI) ELISA

    Immobilized PE-Labeled Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein (Cat. No. HLV-HP2E4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) with a linear range of 0.03-1 ng/mL (QC tested).

    Protocol
  •  HLA-A*0201 & B2M & HBV (FLLTRILTI) ELISA

    Immobilized PE-Labeled Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein (Cat. No. HLV-HP2E4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.03-1 ng/mL (Routinely tested).

    Protocol

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Background

The surface antigens (HBsAg) decorating the lipid envelope of HBV are produced in excess during the life cycle of the virus, and its presence in blood indicates current hepatitis B infection. The Human HLA-A*0201 HBV ( FLLTRILTI) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and FLLTRILTI peptide of the HBV.

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