PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein

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Cat. No. / Size
Price
Qty
HLR-HP2H3-25ug
$410.00
HLR-HP2H3-200ug (100ug X 2)
$2350.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    HLA-A*3303 & B2M & EGF R (HVKITDFGR)

  • Source

    PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein (HLR-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*33:03) & Ile 21 - Met 119 (B2M) & HVKITDFGR peptide (Accession # AAA79865.1 (HLA-A*33:03) & P61769-1 (B2M) & HVKITDFGR).

    Predicted N-terminus: Gly 25 & His

    Request for sequence
  • Molecular Characterization

    PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein is assembled by biotinylated monomer and streptavidin.

    Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein is produced by co-expression of HLA-A*33:03 and B2M loaded with EGF R peptide. Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, 50-150 mM NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     HLA-A*3303 & B2M & EGF R (HVKITDFGR) ELISA

    Immobilized PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein (Cat. No. HLR-HP2H3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (Routinely tested).

    Protocol

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Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The Human HLA-A*3303 & B2M & EGF R(HVKITDFGR) protein is a complex of HLA-A*3303 of the MHC Class I, B2M, and HVKITDFGR peptide of the EGF R.

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