Human ICAM-1 / CD54 Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
IC1-H52H5-100ug
$415.00
IC1-H52H5-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ICAM1, BB2, CD54, P3.58

  • Source

    Human ICAM-1, His Tag (IC1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # P05362-1).

    Predicted N-terminus: Gln 28

    Request for sequence
  • Molecular Characterization

    ICAM-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 51.39 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ICAM-1 SDS-PAGE

    Human ICAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    ICAM-1 SEC-MALS

    The purity of Human ICAM-1, His Tag (Cat. No. IC1-H52H5) is more than 95% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.

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Background

Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.

Recent Advances

 
Drug Development Progress
  • English Name:

    Intercellular adhesion molecule-1

  • Category:

  • Approved Drugs:

    2 Details

  • Drugs in Clinical Trials:

    4 Details

  • Highest Development Stage:

    Approved

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