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Product Details
Synonyms
IFN-alpha 1, IFNA1, IFNalpha 1, IFN-alpha-1, LeIF D
Source
Human IFN-alpha 1, Fc Tag (IFA-H5258) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IFN-alpha 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) is more than 95% and the molecular weight of this protein is around 90-118 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) at 5 μg/mL (100 μL/well) can bind Cynomolgus IFN-alpha/beta R1 Protein, His Tag (Cat. No. IF1-C52H3) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (Cat. No. IF1-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 0.177 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IFN-alpha / beta R1, His Tag (Cat. No. IF1-H5225) on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human IFNAR2, His Tag (Cat. No. IF2-H5224) on HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
m***n2023-06-02
Likesproduct worked well for the desired purpose. IFNa was used as a cytokine control in an elisa. Acro was recommended to me by a colleague who recently purchased another kit.
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