Biotinylated Human IFN-beta Protein, His,Avitag™ (MALS & SPR verified)

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Cat. No. / Size
Price
Qty
IFB-H82E4-25ug
$360.00
IFB-H82E4-200ug
$1420.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IFNB1, Interferon beta, IFN-beta, IFB, IFNB

  • Source

    Biotinylated Human IFN-beta Protein, His,Avitag (IFB-H82E4) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Asn 187 (Accession # P01574).

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  • Molecular Characterization

    IFN-beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 23.9 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IFN-beta SDS-PAGE

    Biotinylated Human IFN-beta Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IFN-beta SEC-MALS

    The purity of Biotinylated Human IFN-beta Protein, His,Avitag (Cat. No. IFB-H82E4) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

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  • Bioactivity-SPR

     IFN-beta SPR

    Biotinylated Human IFN-beta Protein, His,Avitag (Cat. No. IFB-H82E4) captured on Biotin CAP-Series S Sensor Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 203 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Interferon beta(IFN beta) is type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity)

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Drug Development Progress
  • English Name:

    Interferon beta

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    6 Details

  • Highest Development Stage:

    Approved

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