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Product Details
Synonyms
IFNW1, IFN-omega
Source
Human IFN-omega Protein, His Tag (IFA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Ser 195 (Accession # P05000).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.9 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IFN-omega Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) is more than 90% and the molecular weight of this protein is around 18-30 kDa verified by SEC-MALS.
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Bioactivity-BLI
Loaded Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) on NTA Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 245 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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