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Product Details
Synonyms
IGF-I R / IGF-1 R
Source
Biotinylated Human IGF-I R Protein, His,Avitag (IGR-H83E3) is expressed from CHO cells. It contains AA Glu 31 - Asn 932 (Accession # P08069).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 106.4 kDa. The protein migrates as 35-45 kDa and 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IGF-I R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IGF-I R Protein, His,Avitag (Cat. No. IGR-H83E3) is more than 95% and the molecular weight of this protein is around 220-270 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IGF-I Protein, Fc Tag (Cat. No. IG1-H5263) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I R Protein, His,Avitag (Cat. No. IGR-H83E3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human IGF-I R Protein, His,Avitag (Cat. No. IGR-H83E3) immobilized on SA Chip can bind Human IGF-I Protein, His Tag (Cat. No. IG1-H5245) with an affinity constant of 12.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
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