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Product Details
Synonyms
IGFBP4, IBP-4
Source
Human IGFBP-4 Protein, Fc Tag (IG4-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).
Predicted N-terminus: Asp 22
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IGFBP-4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) is more than 85% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human IGF-I, His Tag (Cat. No. IG1-H5245) at 5 μg/mL (100 μL/well) can bind Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag (Cat. No. IG2-H82F9) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
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