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Product Details
Synonyms
IBP7, IGFBP7
Source
Biotinylated Human IGFBP-7, Fc,Avitag (IG7-H82F9) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # Q16270-1).
Predicted N-terminus: Asp 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 54.3 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IGFBP-7, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) is more than 95% and the molecular weight of this protein is around 105-128 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 14.3 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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