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Product Details
Synonyms
IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2
Source
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (IL3-H52H6) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1(C208S,C227S,C232S,C259S)).
Predicted N-terminus: His 109
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (Cat. No. IL3-H52H6) is more than 90% and the molecular weight of this protein is around 18-28 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (Cat. No. IL3-H52H6) with a linear range of 0.3-20 ng/mL (QC tested).
Protocol
Immobilized Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (Cat. No. IL3-H52H6) at 1 μg/mL (100 μL/well) can bind Tozorakimab with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Itepekimab on Protein A Biosensor, can bind Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (Cat. No. IL3-H52H6) with an affinity constant of 3.30 nM as determined in BLI assay (ForteBio Octet RED96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (MALS verified) (Cat. No. IL3-H52H6) induced Human IL-1RL1 & IL-1 RAcP (IL-33 receptor) (Luc) HEK293 Reporter Cell. The EC50 for this effect is 0.012 ng/ml (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-33
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
9 Details
- Highest Development Stage：
Phase 3 Clinical
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