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Product Details
Synonyms
IL4R, CD124, IL4RA
Source
Human IL-4 R alpha, His Tag (ILR-H5221) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1).
Predicted N-terminus: Met 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-4 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) is more than 85% and the molecular weight of this protein is around 32-48 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 2-16 ng/mL (QC tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221): Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) binding reactions. The half maximal inhibitory concentration (IC50) is 0.505 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Anti-IL-4Rα Antibody, Human IgG4 captured on Protein A Chip can bind Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) with an affinity constant of 0.267 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) immobilized on CM5 Chip can bind Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) with an affinity constant of 0.0308 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1.
Recent Advances
Drug Development Progress- English Name：
Interleukin-4 receptor subunit alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Approved
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