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Product Details
Synonyms
Integrin alpha V beta 6, ITGAV&ITGB6
Source
Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (IT6-C52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 (ITGAV) & Gly 22 - Pro 709 (ITGB6) (Accession # A0A2K5WCD3-1 (ITGAV) & A0A2K5TZ36-1 (ITGB6)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Cynomolgus Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113.1 kDa (ITGAV) and 79.0 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The non-reducing (NR) protein migrates as 135-145 kDa (ITGAV) & 85-95 kDa (ITGB6) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.2 M Arginine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) is more than 95% and the molecular weight of this protein is around 195-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.2-10 ng/mL (QC tested).
Protocol
Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Fibronectin fragment, premium grade (Cat. No. FIN-H5113) immobilized on CM5 Chip can bind Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) with an affinity constant of 0.946 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-V/beta-6
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
6 Details
- Highest Development Stage：
Phase 2 Clinical
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