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Product Details
Synonyms
CD258 antigen, CD258, HVEM-L, LIGHT, LTg, TNFSF14, TR2, HVEML
Source
Human LIGHT Protein, His Tag (LIT-H5242) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 58.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human LIGHT Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human LIGHT Protein, His Tag (Cat. No. LIT-H5242) is more than 85% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, His Tag (Cat. No. LIT-H5242) with a linear range of 16-63 ng/mL (QC tested).
Protocol
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, His Tag (Cat. No. LIT-H5242) with a linear range of 8-63 ng/mL (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human LIGHT Protein, His Tag (Cat. No. LIT-H5242) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
E***a2021-09-23
LikesWe use this protein as an ELISA standard. We are interested in quantifying soluble LIGHT concentration from human serum. This active form of the protein perfectly binds its receptors and policlonal antibodies. The standard curve obtained is highly reproducible. It was very easy to find all the information in the web of AcroBiosystems and to download the CoA, which was also very descriptive.
Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 14
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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