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Cynomolgus MAdCAM-1 Protein, His Tag, low endotoxin

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Cat. No. / Size
Price
Qty
MAM-C52H3-100ug
$355.00
MAM-C52H3-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    MAdCAM-1, MAdCAM1, Mucosal vascular addressin cell adhesion molecule 1 transcript variant 1

  • Source

    Cynomolgus MAdCAM-1 Protein, His Tag (MAM-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Lys 291 (Accession # XP_045235080.1).

    Predicted N-terminus: Gln 22

    Request for sequence
  • Molecular Characterization

    MAdCAM-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.5 kDa. The protein migrates as 33-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    MAdCAM-1 SDS-PAGE

    Cynomolgus MAdCAM-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     MAdCAM-1 ELISA

    Immobilized Cynomolgus MAdCAM-1 Protein, His Tag (Cat. No. MAM-C52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT7-H82W4) with a linear range of 0.02-0.313 μg/mL (QC tested).

    Protocol

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Background

The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) takes a key role in the endothelial adhesion and migration of lymphocytes to sites of inflammation in inflammatory disease. In vitro and in vivo data indicate that blockade of the adhesion molecule mucosal address in cell adhesion molecule (MAdCAM) pathway decreases leukocyte homing to the gut by inhibiting the interaction between MAdCAM and its ligand the α4β7 integrin expressed on lymphocytes.

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Drug Development Progress
  • English Name:

    Mucosal addressin cell adhesion molecule-1

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    4 Details

  • Highest Development Stage:

    Approved

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