Product Details
Application
- Cell based assay (FACS or functional assay)
- Affinity testing (SPR / BLI)
- Screening (ELISA)
Synonyms
MSP1D1
Source
Nanodisc PE-Labeled Human MSP1D1 Protein, His Tag (APO-HP1E3) is produced via site-specific conjugation of PE to Human MSP1D1 Protein, His Tag under optimal conditions with a proprietary technology. Human MSP1D1 Protein, His Tag is expressed from E. coli system. It contains AA Ser 1 - Gln 211 (Accession # P11836-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa.
Structure

Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS

5e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 1:20 dilution (5 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human MSP1D1 Protein, His Tag (Cat. No. APO-HP1E3) and negative control protein respectively. PE-SA was used as negative control (QC tested).
Protocol
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