Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag

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Cat. No. / Size
Price
Qty
NP2-V52H3-100ug
$350.00
NP2-V52H3-1mg
$2680.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Nucleocapsid protein/NP (Influenza Virus)

  • Source

    Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag (NP2-V52H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Asn 498 (Accession # EPI1884976, GISAID).

    Predicted N-terminus: Met 1

    Request for sequence

  • Molecular Characterization

    Nucleocapsid protein/NP (Influenza Virus) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.8 kDa. The protein migrates as 55-64 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Nucleocapsid protein/NP (Influenza Virus) SDS-PAGE

    Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Nucleocapsid protein/NP (Influenza Virus) ELISA

    Immobilized Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag (Cat. No. NP2-V52H3) at 1 μg/mL (100 μL/well) can bind Pan Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.06-2 ng/mL (QC tested).

    Protocol

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Background

Influenza, commonly known as ‘the flu’, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses.Influenza viral nucleoprotein (NP) is highly conserved and the most abundant non-enzymatic viral protein in infected cells. NP is a key component of the viral ribonucleoproteins (vRNPs) complex, and its recognized functions include, but are not limited to, binding to RNA and oligomerizing for the vRNP complex, undergoing intracellular trafficking, and participating in the switch from mRNA transcription to vRNA replication.

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