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Product Details
Synonyms
PADI2, PAD-H19, KIAA0994, PAD2, PDI2
Source
Human PADI2, His Tag (PA2-H5543) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Pro 665 (Accession # Q9Y2J8-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 77.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human PADI2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human PADI2, His Tag (Cat. No. PA2-H5543) at 1 μg/mL (100 μL/well) can bind Human PADI2 Antibody-Polyclonal Sheep IgG with a linear range of 0.004-1 μg/mL (QC tested).
Protocol
Bioactivity
Measured by its ability to catalyze the deamination of benzoyl-arginine ethyl ester. The specific activity is >1200 pmol/min/μg (QC tested).
Protocol
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