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Product Details
Synonyms
PDCD1, PD1, CD279, SLEB2
Source
Human PD-1 Protein, Fc Tag (PD1-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.1 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 24 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human PD-1 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) is more than 95% and the molecular weight of this protein is around 105-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc,Avitag (Cat. No. PD2-H82F6) with a linear range of 0.5-16 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human PD-L2, Avitag,His Tag (Cat. No. PD2-H82E8) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
Serial dilutions of nivolumab were added into Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).
Protocol
Immobilized Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL, add increasing concentrations of Ivonescimab and then add Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) at 0.1 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 3.16 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) on ProteinA Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 5.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) on Protein A Biosensor, can bind Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) with an affinity constant of 0.45 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
Flow Cytometry assay shows that Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***22019-10-12
LikesI used this protein for ELISA experiments, coated it in a well plate, and then incubated it with the antibody molecules that were screened. I got very good binding data, and the repeatability and stability are also good.- 1***52019-10-11 LikesThis protein has extremely good affinity and is pretty stable. We dissolved it under the recommended conditions, and the difference between different batches is very small. In the ELISA experiment, it is reproducible whether it is used for coating or as standard. We will continue using it in the future, and will try other proteins as well.
Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
22 Details
- Drugs in Clinical Trials：
193 Details
- Highest Development Stage：
Approved
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