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Product Details
Synonyms
Prefusion glycoprotein F0/pre-F protein (RSV)
Source
HRSV (B) Pre-fusion glycoprotein F0, His Tag (RSF-V52H8) is expressed from human 293 cells (HEK293).
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.5 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
HRSV (B) Pre-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) is more than 90% and the molecular weight of this protein is around 190-240 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) with a linear range of 0.008-0.25 μg/mL (QC tested).
Protocol
Immobilized Recombinant Human Anti-RSV Antibody (AM14), Human IgG1 at 1 μg/mL (100 μL/well) can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
Immobilized Nirsevimab, Human IgG1 kappa at 1 μg/mL (100 μL/well) can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 0.766 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-HRSV-A F/Fusion glycoprotein F0 Antibody, Human IgG1 (D25) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 0.792 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (4G2) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 6.69 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-RSV-Pre-F0 specific Antibody, Human IgG1 (12C6) (Cat. No. RS0-S286) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 2.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (3B4) captured on CM5 chip via anti-mouse antibodies surface can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 2.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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FAQ
- Product
What do “pre” and “post” refer to in the names of viral proteins?
In virology, “pre” refers to the conformation of a viral surface glycoprotein before fusion with the host cell membrane, while “post” refers to the conformation after membrane fusion and entry into the host cell. Both are naturally occurring states in the viral life cycle. Because the “pre” conformation represents a critical stage before viral entry, antibodies designed against this state often exhibit stronger neutralizing activity and are therefore more effective in preventing infection.
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