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Product Details
Synonyms
PTK7, CCK4, CCK-4, Tyrosine-protein kinase-like 7, Protein-tyrosine kinase 7, Protein-tyrosine kinase 7
Source
Human PTK7, His Tag (PT7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).
Predicted N-terminus: Ala 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 76.5 kDa. The protein migrates as 100-125 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human PTK7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PTK7, His Tag (Cat. No. PT7-H52H3) is more than 95% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PTK7, His Tag (Cat. No. PT7-H52H3) at 1 μg/mL (100 μL/well) can bind Cofetuzumab Pelidotin with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cofetuzumab captured on Protein A Chip can bind Human PTK7, His Tag (Cat. No. PT7-H52H3) with an affinity constant of 5.86 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***62022-08-09
LikesValidated by SPR, measured monovalent affinity, anti-human FC chip, loaded diluted antibody, added diluted Folate R, His tag, with the affinity constant of 4 nM.
Background
Recent Advances
Drug Development Progress- English Name：
Protein-tyrosine kinase 7
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Phase 2 Clinical
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