Save Product
Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Human SIRP alpha (31-149), Llama IgG2b Fc Tag (SIA-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Ser 149 (Accession # P78324-1).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 40.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human SIRP alpha (31-149), Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) is more than 90% and the molecular weight of this protein is around 73-98 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
19 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
