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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Human SIRP alpha, Mouse IgG1 Fc Tag (SIA-H52A8) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 63.0 kDa. The protein migrates as 70-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human SIRP alpha, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) is more than 95% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/mL. IC50=0.1318 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
19 Details
- Highest Development Stage：
Approved
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