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Product Details
Synonyms
DDPAC, FTDP-17, MAPT, MSTD, MTBT1, Tau, PHF-tau, TAU
Source
Human Tau-441 (P301S), His Tag (TAU-H5145) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8 (P301S)).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.7 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Tau-441 (P301S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human Tau-441 (P301S), His Tag (Cat. No. TAU-H5145) is more than 85% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human Tau-441 (P301S), His Tag (Cat. No. TAU-H5145) at 1 μg/mL (100 μL/well) can bind Tau Monoclonal Antibody with a linear range of 0.4-13 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Microtubule-associated protein tau
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
46 Details
- Highest Development Stage：
Approved
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