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Product Details
Synonyms
TIGIT, VSIG9, VSTM3
Source
Human TIGIT, His Tag (TIT-H52H5) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.8 kDa. The protein migrates as 30-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TIGIT, His Tag (Cat. No. TIT-H52H5) is more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H5) at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H5) at 1 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.078-5 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-hTIGIT antibody captured on CM5 chip via Anti-mouse antibodies surface can bind Human TIGIT, His Tag (Cat. No. TIT-H52H5) with an affinity constant of 1.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) on SA Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H5) with an affinity constant of 1.9 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T cell immunoglobulin and ITIM domains
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
41 Details
- Highest Development Stage：
Phase 3 Clinical
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