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Human TIM-3 / HAVCR2 Protein, Mouse IgG2a Fc Tag, low endotoxin

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Cat. No. / Size
Price
Qty
TM3-H525b-100ug
$360.00
TM3-H525b-1mg (500ug X 2)
$2495.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

  • Source

    Human TIM-3 Protein, Mouse IgG2a Fc Tag (TM3-H525b) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).

    Predicted N-terminus: Ser 22

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  • Molecular Characterization

    TIM-3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 46.8 kDa. The protein migrates as 50-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    TIM-3 SDS-PAGE

    Human TIM-3 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     TIM-3 ELISA

    Immobilized Human TIM-3 Protein, Mouse IgG2a Fc Tag (Cat. No. TM3-H525b) at 0.2 μg/mL (100 μL/well) can bind Anti-TIM3 MAb, Human IgG4 with a linear range of 0.2-2 ng/mL (QC tested).

    Protocol

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Background

Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Recent Advances

 
Drug Development Progress
  • English Name:

    Hepatitis A virus cellular receptor 2

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    23 Details

  • Highest Development Stage:

    Phase 3 Clinical

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