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Product Details
Synonyms
CO-029, tetraspanin 8, TM4SF3, TM4SF3tspan-8, Transmembrane 4 superfamily member 3tetraspanin-8, TSPAN8, Tspan-8, Tumor-associated antigen CO-029
Source
Human TSPAN8, His Tag (TS8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 110 - Asn 205 (Accession # P19075).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 12.9 kDa. The protein migrates as 14 kDa and 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human TSPAN8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TSPAN8, His Tag (Cat. No. TS8-H52H3) is more than 95% and the molecular weight of this protein is around 13-20 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TSPAN8, His Tag (Cat. No. TS8-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-TSPAN8 mIgG2b Antibody with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Anti-TSPAN8 mIgG2b Antibody at 1 μg/mL (100 μL/well) can bind Human TSPAN8, His Tag (Cat. No. TS8-H52H3) with a linear range of 4-63 ng/mL (Routinely tested).
Protocol
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