This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD40 Protein, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD40 Protein, Fc Tag (MALS verified) (Cat. No.CD0-H5253) was more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Loaded Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS analysis shows that Human CD40, Fc Tag (HPLC-verified) (Cat .No. CD0-H5253) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.1 μg/mL (Routinely tested).
Authors: Baeuerle Patrick, et al
Journal: US20200148771A1 2020
Authors: Yan Shude , et al
Journal: US20200148778A1 2020
Application: ELISA & Immunization & SPR
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Price(USD) : $290.00
Price(USD) : $1980.00
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