0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > CD40 > CD0-H5253

Human CD40 / TNFRSF5 Protein, Fc Tag (MALS verified)

  • Synonym
    CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50
  • Source
    Human CD40 Protein, Fc Tag (MALS verified) (CD0-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Arg 193) P25942-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 45.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. CD0-H5253) SDS-PAGE gel

Human CD40 Protein, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. ) MALS images

The purity of Human CD40 Protein, Fc Tag (MALS verified) (Cat. No.CD0-H5253) was more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD40 Protein, Fc Tag (MALS verified)Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. CD0-H5253) ELISA bioactivity

Immobilized Human CD40 Ligand, His,Flag Tag (active trimer) (Cat. No. CDL-H52Db) at 5 μg/mL (100 μL/well) can bind Human CD40, Fc Tag (Cat. No. CD0-H5253) with a linear range of 0.02-0.156 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Human CD40, Fc Tag (HPLC-verified) (Cat .No. CD0-H5253) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.1 μg/mL (Routinely tested).

Comments (0)

Order Details

Price(USD) : $290.00

Price(USD) : $1980.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:27 Details
  • Latest Research Phase:Phase ?

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop