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Your Position: Home > Protein-coupled magnetic beads > EGF R > MBE-K012

EGFR-coupled magnetic beads

For research use only.
Materials Provided
Items Size Size
Beads Size 2 mg 10 mg
Particle size 2 μm 2 μm
Beads Surface hydrophilic hydrophilic
Amount of protein coupled >300nmol /mg Beads >300nmol /mg Beads
Binding Capacity > 200 nmol antibody/ mg beads > 200 nmol antibody / mg beads
Formulation Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose
Reconstitution 2 mL ultrapure water (1mg beads/mL) 10 mL ultrapure water (1mg beads/mL)
  • Background
    The biotinylated EGFR protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-EGFR antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This EGFR coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

    An immediate use is highly recommended after reconstitution.

    Do not to freeze thaw the Beads after reconstitution.

  • Assay Principles
    The conjugation was achieved by means of the binding between streptavidin and biotin. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L. Thus, the binding of streptavidin and biotin is irreversible. Our EGFR pre-coupled beads could capture anything binding to EGFR, and make the following testing easy, such as cell stimulating, biopanning or flow cytometric analysis.
    Application Method:
    • Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.

    • Add the prepared beads to your samples.

    • Beads can be separated from your samples afterwards using a magnetic plate.

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