Product Details
Source
Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) is a neutralizing rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.
Antibody Type
Recombinant Monoclonal
Clone
1G10
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-FACS

Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with APC-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (Cat. No. FM3-AFM721) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).
Protocol
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