Save Product
Product Details
Source
APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 is a neutralizing antibody produced via site-specific conjugation of APC to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology. We also carry another proclin-containing version of APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-AY54A1) produced with the same production process except for proclin, and the proclin-containing product has the same performance and can be stored under 2-8 °C for no less than 12 months after reconstitution.
Clone
Y45
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.5% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
Customers Also Viewed
Performance Data
Bioactivity-FACS
5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-AY54P1) and isotype control antibody respectively. APC signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-AY54P1) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 μL), washed and then analyzed with FACS. Both FITC and APC positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
