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Product Details
Source
Rabbit Anti-Mouse FMC63 Polyclonal Antibody is a polyclonal antibody purified from rabbit serum.
Antibody Type
Polyclonal Antibody
Host Species
Rabbit
Reactivity
Mouse
Immunogen
FMC63 scFv
Specificity
Recognizes FMC63 scFv specifically.
Application
ApplicationRecommended UsageELISA1. 1:15000-1:300000 2. 1:50-1:500Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).
Protocol
A microplate was coated with FMC63 monoclonal antibody, Mouse IgG2a, at 1 μg/mL. Serially diluted Rabbit Anti-Mouse FMC63 Polyclonal Antibody was added, followed by Biotinylated FMC63 scFv-Fc-Avitag at 2 μg/mL. Binding was detected using HRP-conjugated streptavidin. The QC-tested sensitivity was 78 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
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