Product Details
Source
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
Y45
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
FMC63
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived antibody.
Application
ApplicationRecommended UsageELISA1:2000-1:20000Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 6 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) with a linear range of 0.1-6 ng/mL. FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is verified not recoginized by Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) in low concentration (QC tested).
Protocol
ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) was inhibited by increasing concentration of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).
Protocol
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