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Product Details
Application
Synonyms
Claudin-18.2, CLDN18, Claudin-18
Source
Nanodisc-pro Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (CL2-H5589) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2). In the region Met 1 - Ala 200, the amino acid sequences of Human and Cynomolgus Claudin-18.2 are identical.
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, and a twin-strep tag at the C-terminus. The protein has a calculated MW of 28.5 kDa.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".
Synthesis Process
Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Cat. No. CL2-H5589) at 2 μg/mL (100 μL/well) can bind Anti-Claudin-18.2 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 captured on Protein A Chip can bind Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Cat. No. CL2-H5589) with an affinity constant of 29.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Cat. No. CL2-H5589) on NTA Biosensor, can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 (AM34) with an affinity constant of 2.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of Anti-Claudin-18.2 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Nanodisc-pro) (Cat. No. CL2-H5589) and isotype control protein respectively, washed and then followed by PE anti-His Tag Antibody and analyzed with FACS (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Claudin-18 splice variant 2
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
82 Details
- Highest Development Stage：
Approved
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