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Product Details
Synonyms
FCGR1A, FCG1, FCGR1, IGFR1, CD64, CD64A, FCRI
Source
Cynomolgus CD64, His Tag (FCA-C52H6) is expressed from human 293 cells (HEK293). It contains AA Val 11 - Pro 288 (Accession # NP_001270969.1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.2 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD64, His Tag (Cat. No. FCA-C52H6) is more than 95% and the molecular weight of this protein is around 35-52 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus CD64, His Tag (Cat. No. FCA-C52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Cynomolgus CD64, His Tag (Cat. No. FCA-C52H6) captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 4.43 nM as determined in SPR assay (Biacore T200) (QC tested).
Protocol
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Background
High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
Recent Advances
Drug Development Progress- English Name：
High affinity immunoglobulin gamma Fc receptor I
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
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