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Product Details
Synonyms
IL12, p70, Interleukin-12
Source
Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (IL2-R52H9) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 & Arg 23 - Ser 219 (Accession # P48095 & P48091).
Predicted N-terminus: Ile 23 & Arg 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag, produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.7 kDa (IL-12B) and 24.4 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, and subunit IL-12A contains a polyhistidine tag at the C-terminus. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (Cat. No. IL2-R52H9) is more than 90% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (Cat. No. IL2-R52H9) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Immobilized Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (Cat. No. IL2-R52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (Routinely tested).
Protocol
Immobilized Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (Cat. No. IL2-R52H9) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (Cat. No. ILB-C52E2) with a linear range of 2-78 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-12
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
35 Details
- Highest Development Stage：
Approved
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