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Product Details
Synonyms
IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
Source
Cynomolgus IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (ILR-C52H9) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 & Ala 25 - Thr 341 (Accession # G7Q0S7 & XP_005594500.1).
Predicted N-terminus: Met 26 & Ala 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Cynomolgus IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag, produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 29.0 kDa (IL-4 R alpha) and 41.7 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, and subunit IL-13 R alpha 1 contains a polyhistidine tag at the C-terminus. The protein migrates as 40-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-C52H9) is more than 90% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus IL-4, Fc Tag (Cat. No. IL4-C5259) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-C52H9) with a linear range of 1-39 ng/mL (QC tested).
Protocol
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