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Product Details
Synonyms
IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
Source
Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (ILR-H82W3) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & P78552-1).
Predicted N-terminus: Met 26 & Gly 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 32.1 kDa (IL-4 R alpha) and 43.6 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-13 R alpha 1 is fused with a polyhistidine tag at the C-terminus. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (Cat. No. ILR-H82W3) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (Cat. No. ILR-H82W3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) with a linear range of 0.2-5 ng/mL (QC tested).
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