Product Details
Synonyms
IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
Source
Mouse IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (ILR-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 233 & Ala 26 - Thr 340 (Accession # NP_001008700 & NP_598751.3).
Predicted N-terminus: Ile 26 & Ala 26
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
Mouse IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag, produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 29.6 kDa (IL-4 R alpha) and 41.2 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, and subunit IL-13 R alpha 1 contains a polyhistidine tag at the C-terminus. The protein migrates as 38-43 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Mouse IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Mouse IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-M52H8) is more than 85% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Mouse IL-4, His Tag (Cat. No. IL4-M52H5) immobilized on CM5 Chip can bind Mouse IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-M52H8) with an affinity constant of 0.843 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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